Journal: Frontiers in Immunology
Article Title: Nanospheres as the delivery vehicle: novel application of Toxoplasma gondii ribosomal protein S2 in PLGA and chitosan nanospheres against acute toxoplasmosis
doi: 10.3389/fimmu.2024.1475280
Figure Lengend Snippet: Flow cytometry analysis of CD4 + (A) and CD8 + T lymphocytes (B) in splenic lymphocytes harvested from immunized mice. Five mice in each group were sacrificed, and spleen lymphocytes from each animal were investigated. The dot plots (C) showed the relative proportion of CD3e + CD4 + and CD3e + CD8 + cells. Values are shown as mean ± SD (n = 5), and significance was evaluated by one-way ANOVA followed by Dunnett’s test. Values among the TgRPS2 protein, TgRPS2-PLGA, and TgRPS2-CS groups were pairwise compared by ANOVA following Bonferroni’s correction. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with control group.
Article Snippet: The spleen lymphocytes were, respectively, stained with anti-mouse CD3e-PE and CD4-FITC (eBioscience, San Diego, CA, USA), and anti-mouse CD3e-PE and CD8-FITC (eBioscience, San Diego, CA, USA) in the dark for 40 minutes at 4°C.
Techniques: Flow Cytometry, Control